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Anti-AKT Rabbit mAb [69K96M86]

Purified Rabbit Monoclonal Antibody

货号 规格 价格
R011234
100µL ¥1780.00
50µL ¥1080.00
20µL ¥588.00

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using AKT1/2/3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of AKT in 3T3, mouse lung lysates using AKT antibody.

Western blot analysis of AKT1/2/3 in Jurkat, rat Brain, C6, CHO-K1, Hela lysates using AKT antibody.

Product Name Anti-AKT Rabbit mAb
Description Purified Rabbit Monoclonal Antibody
Application
  • Applications Legend:
  • WB=Western Blotting
  • IHC-P=Immunohistochemistry (Paraffin)
  • IHC-F=Immunohistochemistry (Frozen)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-F, IHC-P, ICC/IF, IP, ELISA
Dilution WB 1:500~1:1,000; IHC-P 1:50~1:100; IF 1:50~1:200; IP 1:20
Reactivity Human, Mouse, Rat, Hamster
Host Rabbit
Clonality Monoclonal
Clone No. 69K96M86
Isotype IgG
Target / Specificity Recombinant protein of human AKT1/2/3
Format Buffer System: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA Purification: Affinity Purified.
Gene ID 207/208/10000
Other Names AKT1.
Calculated MW Calculated MW: 56 kDa; Observed MW: 56 kDa
Primary Accession P31749/P31751/Q9Y243
Storage Shipped at 4℃. Upon delivery aliquot. Store at 4℃ short term (1~2 weeks). Store at -20℃ for 2 years. Avoid freeze / thaw cycles.
Precautions Anti-AKT antibody [69K96M86] is for research use only and not for use in diagnostic or therapeutic procedures.
Background Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.

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