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Anti-Cytokeratin 19 Rabbit pAb

Affinity Purified Rabbit Polyclonal Antibody

货号 规格 价格
P900001
100µL ¥1780.00
50µL ¥1080.00
20µL ¥588.00

Immunofluorescent analysis of paraffin-embedded human lung cancer tissue using Anti-Cytokeratin 19 Rabbit pAb. Antigen repair using EDTA antigen repair solution and blocking with Goat serum for half 0.5 hour. Samples were incubated with primary antibody (1/1000) overnight at 4℃. A undiluted Dylight 488 Fluorescein-labeled anti-rabbit IgG was used as the secondary antibody for 0.5 hour.

All lanes: Anti-Cytokeratin 19 Rabbit pAb at 1:1,000 dilution

Lane 1: 293T cell Lysates

Lane 2: Hela cell Lysates

Lane 3: HepG2 cell Lysates

Lysates/proteins at 20 µg per lane.

Secondary Goat Anti-Rabbit IgG, (H L), Peroxidase conjugated (LF102) at 1:2,000 dilution.

Observed band size: 44 kDa

Blocking/Dilution buffer: 1×PBST.

All lanes: Anti-Cytokeratin 19 Rabbit pAb at 1:1,000 dilution

Lysates/proteins at 20 µg per lane.

Secondary Goat Anti-Rabbit IgG, (H L), Peroxidase conjugated (LF102) at 1:5,000 dilution.

Observed band size: 44 kDa

Blocking/Dilution buffer: 1×PBST.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using Anti-Cytokeratin 19 Rabbit pAb. Antigen repair using EDTA antigen repair solution and blocking with 5% BSA for half 0.5 hour. Samples were incubated with primary antibody (1/1000) overnight at 4℃. A undiluted HRP-labeled anti-rabbit IgG was used as the secondary antibody for 0.5 hour.

Product Name Anti-Cytokeratin 19 Rabbit pAb
Description Affinity Purified Rabbit Polyclonal Antibody
Application
  • Applications Legend:
  • WB=Western Blotting
  • IHC-P=Immunohistochemistry (Paraffin)
  • IHC-F=Immunohistochemistry (Frozen)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF, ELISA
Dilution WB 1:1,000; IHC-P 1:1,000; IF 1:1,000
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target / Specificity This Cytokeratin 19 antibody is generated from rabbits immunized with a BSA conjugated synthetic peptide between 384-398 amino acids from the C-terminal region of human Cytokeratin19.
Format Purified polyclonal antibody supplied in PBS. This antibody is purified through a peptide affinity column.
Gene ID 3880
Other Names KRT19, Keratin, type I cytoskeletal 19, Cytokeratin-19, CK-19, CK19, Keratin-19, K1CS, keratin 19, K19.
Calculated MW 44 kDa
Primary Accession P08727
Other Accession NP_002267.2
Antigen Region 384-398aa
Storage Shipped at 4℃. Upon delivery aliquot. Store at 4℃ short term (1~2 weeks). Store at -20℃ for 2 years. Avoid freeze / thaw cycles.
Precautions Anti-Cytokeratin 19 Rabbit pAb is for research use only and not for use in diagnostic or therapeutic procedures.
Background Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Tissue Location Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.

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