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Anti-PDIA1 (PDI) Rabbit pAb

Affinity Purified Rabbit Polyclonal Antibody

货号 规格 价格
P900003
100µL ¥1780.00
50µL ¥1080.00
20µL ¥588.00

Immunohistochemical analysis of paraffin-embedded uterine tissue using Anti-PDI Rabbit pAb. Antigen repair using EDTA antigen repair solution and blocking with 5% BSA for half 0.5 hour.Samples were incubated with primary antibody (1/1000) overnight at 4℃. A undiluted HRP-labeled anti-rabbit IgG was used as the secondary antibody for 0.5 hour.

All lanes: Anti-PDIA1 (PDI) Rabbit pAb at 1:5,000 dilution

Lane 1: 293T cell Lysates

Lane 2: Hela cell Lysates

Lane 3: HepG2 cell LysatesLysates/proteins at 20 µg per lane.

Secondary Goat Anti-Rabbit IgG, (H L), Peroxidase conjugated (LF102) at 1:2,000 dilution. Observed band size: 57 kDaBlocking/Dilution buffer: 1×PBST.

Product Name Anti-PDIA1 (PDI) Rabbit pAb
Description Affinity Purified Rabbit Polyclonal Antibody
Application
  • Applications Legend:
  • WB=Western Blotting
  • IHC-P=Immunohistochemistry (Paraffin)
  • IHC-F=Immunohistochemistry (Frozen)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, ELISA
Dilution WB 1:5,000; IHC-P 1:1,000
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target / Specificity This PDI antibody is generated from rabbits immunized with a BSA conjugated synthetic peptide between 488-502 amino acids from the C-terminal region of human PDI.
Format Purified polyclonal antibody supplied in PBS. This antibody is purified through a protein G column.
Gene ID 5034
Other Names P4HB,ERBA2L, PDI, PDIA1, PO4DB
Calculated MW 57 kDa
Primary Accession P07237
Other Accession NP_000909.2
Antigen Region 488-502aa
Storage Shipped at 4℃. Upon delivery aliquot. Store at 4℃ short term (1~2 weeks). Store at -20℃ for 2 years. Avoid freeze / thaw cycles.
Precautions Anti-PDIA1 (PDI) Rabbit pAb is for research use only and not for use in diagnostic or therapeutic procedures.
Background This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.
Cellular Location Endoplasmic reticulum. Endoplasmic reticulum lumen. Melanosome. Cell membrane; Peripheral membrane protein. Note=Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources (Probable). Localizes near CD4-enriched regions on lymphoid cell surfaces (PubMed:11181151). Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:10636893) Colocalizes with MTTP in the endoplasmic reticulum (PubMed:23475612) {ECO:0000269|PubMed:10636893, ECO:0000269|PubMed:11181151, ECO:0000269|PubMed:23475612, ECO:0000305}.
Tissue Location Stromal cell of endometrium, parotid gland, body of pancreas, jejunal mucosa, ileal mucosa, islet of Langerhans, right lobe of liver, duodenum, endometrium epithelium, lower esophagus mucosa, adenohypophysis, type B pancreatic cell, minor salivary gland, left adrenal gland, left adrenal gland cortex, right adrenal gland.

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